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Botryllus tuberatus (Ritter & Forsyth, 1917)

Pacific Star Tunicate

Adeline Ang (2014)



Fact Sheet

Brief Summary

Physical Description

External Features

Internal Features

Slides Preparation


Life History & Behaviour

Feeding Mechanisms


Internal Transport & Movement

Asexual Reproduction

Sexual Reproduction

Anatomy & Physiology

Evolution & Systematics

Biogeographic Distribution

Biofouling Threats

References & Links

Slides Preparation

Two colonies of B. tuberatus were fixed and sent in for sectioning on 14thMay 2014, wednesday.  The first colony had 40-50 transverse sections (figure 1) while the second colony had 40-45 longitudinal sections (figure 2).  They were both stained with Hematoxylin and Eosin (H&E).  Hematoxylin is a blue-violet/brown nuclear stain while eosin is a counterstain to hematoxylin and stains red blood cells, cytoplasmic material, cell membranes and extracellular structures pink or red. 

Figure 1.  Image showing an example of a transverse section from B. tuberatus with the H&E stains.

Figure 2.  Image showing an example of a longitudinal section from B. tuberatus with the H&E stains.